PENETAPAN KADAR SIANIDA PADA BIJI KLUWAK (Pangium edule Reinw) MENGGUNAKAN SPEKTROFOTOMETER UV-Vis

Grace Angel Modesta, Rembe (2024) PENETAPAN KADAR SIANIDA PADA BIJI KLUWAK (Pangium edule Reinw) MENGGUNAKAN SPEKTROFOTOMETER UV-Vis. Other thesis, Universitas Setia Budi.

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Abstract

Kluwak seeds, used as the main spice that imparts the distinctive black color, aroma, and taste to rawon broth, contain active compounds such as cyanide, hydrocarpic acid, khaulmograt acid, gorlat acid, and tannins. Cyanide, which is found in kluwak seeds, is a toxic compound that can pose a health risk to humans if consumed in excess. This study aims to determine the cyanide content in kluwak seeds.This research is experimental, aiming to measure the cyanide content in kluwak seeds. Cyanide analysis was performed qualitatively using FeCl3 and AgNO3 reagents, resulting in an orange color and a white precipitate. For quantitative analysis, UV-Vis spectrophotometry was used with a standard cyanide solution, ninhydrin reagent, sodium carbonate, and sodium hydroxide. The cyanide content was calculated using linear regression, at a wavelength of 597 nm. The results showed that the cyanide content in the examined kluwak seeds, measured using UV-Vis spectrophotometry, was 9.08 ppm. INTISARI Biji kłuwak digunakan sebagai bumbu utama yang memberikan warna hitam, aroma dan rasa khas pada kuah rawon. Biji kluwak mengandung senyawa antioksidan diantaranya terdapat sianida, asam hidrokarpat, asam khaulmograt. asam gorlat dan tannin. Kadar sianida dapat membahayakan dan cukup beracun bagi manusia jika dikonsumsı, Tujuan penelitian ini adalah Untuk mengetahui kadar kandungan sianida yang ada dalam biji kluwak (Penguin edule Reinw). Jenis penelitian ini adalah experimental yang bertujan mengetahui kadar sianida pada bijı kluwak. Analisis sianida pada bji kluwak dilakukan secara kualitatif menggunakan pereaksi FeCl3 dan AgNO3 menghasilkan warna orange dan endapan putih. Analisis kuntitatif menggunakan metode spektrofotometri Uv-Vis dengan larutan standar sianida, pereaksi ninhydrin, natrium karbonat, dan natrium hidroksida. Perhitungan kadar sianida menggunakan regrasi linier pada panjang gelombang 597 nm. Hasil penelitian menunjukan kandungan sianida pada biji kluwak yang diperiksa menggunakan metode spektrofotometer UV-Vis sebesar 9,08 ppm.
Item Type: Thesis (Other)
Uncontrolled Keywords: Kluwak Seeds, Cyanide, Uv-Vis Spectrophotometer Biji Kluwak, Sianida, Spektrofotometer UV-Vis
Subjects: Q Science > QK Botany
R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Universitas Setia Budi > Fakultas Ilmu Kesehatan > D3 Analis Kesehatan
Depositing User: Unnamed user with email baa.si@setiabudi.ac.id
Date Deposited: 02 Sep 2026 04:08
Last Modified: 02 Sep 2026 04:08
URI: https://eprints.setiabudi.ac.id/id/eprint/577

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