UJI AKTIVITAS DAN FORMULASI SEDIAAN ESSENCE DARI Saccharomyces Rice Ferment Filtrate (SRFF) SEBAGAI ANTI-AGING SECARA IN VIVO

Saccharomyces Rice Ferment Filtrate (SRFF) merupakan nasi yang difermentasikan menggunakan ragi instan. Filtrat SRFF mengandung fenolik dan alkaloid yang diformulasikan menjadi essence yang yang berpotensi sebagai anti-aging. Penelitian ini bertujuan mengetahui konsentrasi formula essence SRFF terbaik sebagai anti- aging. Proses fermentasi nasi dengan ditambahkan ragi Saccharomyces cerevisiae. Hasil uji mutu fisik fermentasi nasi meliputi, uji organoleptis, pH, dan suhu. Hasil fermentasi dibuat sediaan essence dengan konsentrasi bahan aktif yang digunakan SRFF 5% masa inkubasi 1 hari dan SRFF 10% masa inkubasi 3 hari. Pengujian mutu fisik sediaan essence meliputi uji organoleptis, homogenitas, pH, viskositas, dan stabilitas. Pengujian aktivitas anti-aging sediaan essence pada punggung kelinci New Zealand menggunakan alat skin analyzer dengan parameter konsentrasi kolagen, elastisitas, dan kelembaban. Hasil analisis data mutu fisik sediaan dan anti-aging dianalisis dengan metode ANOVA dan paired t-test. Berdasarkan hasil pengujian mutu fisik sediaan essence, menunjukkan sediaan essence 5% memiliki uji mutu fisik yang baik sesuai rentang syarat uji. Berdasarkan hasil pengujian aktivitas anti- aging menggunakan alat skin analyzer. Konsentrasi yang paling efektif yaitu sediaan essence 5%, karena dengan dosis kecil sudah memberikan aktivitas sebagai anti-aging. Kesimpulan yang didapat pada penelitian ini, sediaan essence 5% memiliki mutu fisik dan aktivitas yang terbaik. Saccharomyces Rice Ferment Filtrate (SRFF) is rice fermented using instant yeast. SRFF filtrate contains phenolics and alkaloids that are formulated into essence which has the potential as anti-aging. This study aims to determine the best concentration of SRFF essence formula as anti-aging. The process of rice fermentation by adding Saccharomyces cerevisiae yeast. The results of the physical quality test of rice fermentation include organoleptic, pH, and temperature tests. The results of fermentation are made essence preparations with the concentration of active ingredients used SRFF 5% incubation period of 1 day and SRFF 10% incubation period of 3 days. Physical quality testing of essence preparations includes organoleptical, homogeneity, pH, viscosity, and stability tests. Testing the anti-aging activity of essence preparations on the back of New Zealand rabbits using a skin analyzer with parameters of collagen concentration, elasticity, and moisture. The results of the physical quality and anti-aging data analysis were analyzed using ANOVA and paired t-test methods. Based on the results of physical quality testing of essence preparations, it shows that 5% essence preparations have a good physical quality test according to the range of test requirements. Based on the results of anti-aging activity testing using a skin analyzer. The most effective concentration is 5% essence preparation, because with a small dose it already provides anti-aging activity. The conclusion obtained in this study, 5% essence preparation has the best physical quality and activity.