Ramadhani, Delfi Fitria (2025) PERBANDINGAN KEMAMPUAN PEREDAMAN RADIKAL BEBAS DPPH (1,1-difenil-2-pikrilhidrazil) AIR REBUSAN TEH KOMBINASI BUNGA TELANG (Clitoria ternatea L.), DAUN TEH HIJAU (Camellia sinensis L. Kuntze), DAN DAUN MINT (Mentha piperita L.). Other thesis, Universitas Setia Budi.
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Abstract
Radikal bebas dapat dihambat oleh antioksidan, yakni zat yang
dapat memperlambat dan mencegah terjadinya oksidasi molekul.
Antioksidan pada teh juga terbukti dapat meningkatkan kapasitas
antioksidan total dalam tubuh. Tidak hanya teh yang terbuat dari rebusan
daun teh, di Indonesia juga banyak teh yang terbuat dari rebusan berbagai
jenis tanaman diantaranya bunga telang (Clitoria ternatea L.), daun teh
hijau (Camellia sinensis L. Kuntze), dan daun mint (Mentha piperita L.).
Tujuan dari penelitian ini adalah untuk mengetahui proporsi dari air
rebusan teh kombinasi bunga telang, daun teh hijau, dan daun mint
memiliki kemampuan peredaman radikal bebas DPPH dan memiliki
kemampuan peredaman radikal bebas terbaik.
Penelitian ini diawali dengan pembuatan simplisia, uji kadar air,
kadar sari larut air, dan kadar sari larut etanol. Lalu air rebusan dibuat
dari teh kombinasi bunga telang, daun teh hijau, dan daun mint dengan
7 proporsi perbandingan. Selanjutnya air rebusan teh diencerkan menjadi
500 ppm dan ditetapkan % peredaman radikal bebas DPPH. Nilai %
peredaman kemudian dianalisis statistik dengan Kolmogorov-Smirnov,
Kruskal Wallis dan Mann Whitney.
Hasil penelitian ini menunjukkan bahwa pada semua proporsi
dari air rebusan teh kombinasi bunga telang, daun teh hijau, daun mint
memiliki kemampuan peredaman radikal bebas DPPH. Proporsi air
rebusan teh dari kombinasi bunga telang, daun teh hijau, dan daun mint
yang memiliki kemampuan peredaman radikal bebas DPPH yang tinggi
adalah daun mint tunggal; kombinasi bunga telang, daun teh hijau, dan
daun mint (1,5:1,0:0,5); dan kombinasi bunga telang, daun teh hijau, dan
daun mint (1,0:1,0:1,0) pada konsentrasi air rebusan 500 ppm.
Free radicals can be inhibited by antioxidants, which are
substances that can slow down and prevent the oxidation of molecules.
Antioxidants in tea have also been proven to increase the total
antioxidant capacity in the body. In Indonesia, not only tea made from
tea leaves is consumed, but also various herbal teas made from different
plants, including butterfly pea flower (Clitoria ternatea L.), green tea
leaves (Camellia sinensis L. Kuntze), and mint leaves (Mentha piperita
L.). The aim of this study was to determine the composition of brewed
tea made from a combination of butterfly pea flower, green tea leaves,
and mint leaves that possesses DPPH free radical scavenging ability and
to identify the combination with the best free radical scavenging activity.
This research began with the preparation of simplicia, followed
by testing for moisture content, water-soluble extract content, and
ethanol-soluble extract content. Then, decoctions were prepared from a
tea combination of butterfly pea flower, green tea leaves, and mint leaves
in seven different composition ratios. The tea infusions were then diluted
to a concentration of 500 ppm, and the percentage of DPPH free radical
scavenging was determined. The percentage inhibition values were then
statistically analyzed using the Kolmogorov-Smirnov, Kruskal-Wallis,
and Mann-Whitney tests.
The results of this study showed that all combinations of the tea
infusion made from butterfly pea flower, green tea leaves, and mint
leaves had DPPH free radical scavenging ability. The combinations with
the best DPPH free radical scavenging ability at a 500 ppm concentration
were: mint leaves alone; a combination of butterfly pea flower, green tea
leaves, and mint leaves in a (1,5:1,0:0,5) ratio; and a combination of
butterfly pea flower, green tea leaves, and mint leaves in a (1,0:1,0:1,0)
ratio.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | air rebusan, antioksidan, radikal bebas, DPPH boiling water, antioxidants, free radicals, DPPH |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Universitas Setia Budi > Fakultas Farmasi > S1 Farmasi |
| Depositing User: | Unnamed user with email baa.si@setiabudi.ac.id |
| Date Deposited: | 22 Dec 2025 06:19 |
| Last Modified: | 22 Dec 2025 06:19 |
| URI: | https://eprints.setiabudi.ac.id/id/eprint/34 |
