UJI AKTIVITAS ANTIJAMUR EKSTRAK JAMUR LING ZHI (Ganoderma lucidum) TERHADAP Candida albicans ATCC 10231

Jamur Ling zhi (Ganoderma lucidum) merupakan jenis jamur obat yang diakui dalam Traditional China Medicine (TCM). Jamur Ling zhi memiliki kandungan senyawa aktif diantaranya flavonoid, alkaloid, tanin dan terpenoid yang bermanfaat sebagai antijamur. Penelitian ini bertujuan untuk menguji aktivitas antijamur ekstrak jamur Ling zhi terhadap Candida albicans ATCC 10231 dan untuk mengetahui nilai KHM dan KBM terhadap Candida albicans ATCC 10231. Penelitian ini termasuk penelitian eksperimental. Ekstrak jamur Ling zhi diperoleh dengan proses maserasi menggunakan etanol 70%. Metode pengujian yang digunakan dalam penelitian ini adalah metode dilusi. Penentuan efektifitas antijamur ekstrak jamur Ling zhi terhadap jamur uji Candida albicans metode dilusi dengan seri konsentrasi 0,15%, 0,31%, 0,62%, 1,25%, 2,5%, 5%, 10%, 20%, 40%, 80%. Hasil penelitian menunjukkan bahwa ekstrak jamur Ling zhi (Ganoderma lucidum) memiliki aktivitas antijamur yang terhadap Candida albicans ATCC 10231. Ekstrak jamur Ling zhi dapat menghambat dan membunuh pertumbuhan jamur Candida albicans. Konsentrasi Hambat Minimum (KHM) dari ekstrak jamur Ling zhi adalah 5% dan Konsentrasi Bunuh Minimum (KBM) terhadap Candida albicans ATCC 10231 adalah 40%. The Lingzhi mushroom (Ganoderma lucidum) is a medicinal fungus recognized in Traditional Chinese Medicine (TCM). Lingzhi mushroom contains active compounds including flavonoids, alkaloids, tannins, and terpenoids, which are beneficial as antifungal agents. This study aimed to test the antifungal activity of Lingzhi mushroom extract against Candida albicans ATCC 10231 and determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) against Candida albicans ATCC 10231. This research employed an experimental design. Lingzhi mushroom extract was obtained through maceration using 70% ethanol. The testing method used in this study was the dilution method. The effectiveness of Lingzhi mushroom extract as an antifungal agent against Candida albicans was determined using dilution series of concentrations: 0,15%, 0,31%, 0,62%, 1,25%, 2,5%, 5%, 10%, 20%, 40%, 80%. The results indicated that Lingzhi mushroom extract (Ganoderma lucidum) exhibited antifungal activity against Candida albicans ATCC 10231. The extract could inhibit and kill the growth of Candida albicans. The Minimum Inhibitory Concentration (MIC) of Lingzhi mushroom extract was 5%, and the Minimum Fungicidal Concentration (MFC) against Candida albicans ATCC 10231 was 40%.