Kristanti, Kristanti (2023) PENGARUH VARIASI SUHU PEMANASAN TERHADAP KADAR AKRILAMIDA PADA UBI JALAR CILEMBU SECARA SPEKTROFOTOMETRI UV - VIS. Other thesis, Universitas Setia Budi.
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Abstract
Akrilamida merupakan senyawa yang bersifat karsinogenik,
mekanisme terbentuk dengan adanya reaksi antara protein (asam amino)
dengan karbohidrat (gula pereduksi) yang tinggi melalui proses
pemanasan suhu tinggi. Ubi jalar cilembu mengandung protein dan
karbohidrat yang tinggi, jika diolah dengan pemanasan suhu tinggi akan
berpotensi terbentuknya akrilamida. Tujuan penelitian ini adalah untuk
mengetahui pengaruh variasi suhu terhadap kadar akrilamida yang
terbentuk pada ubi jalar cilembu.
Sampel ubi jalar cilembu dioven pada variasi suhu 100; 120; 140;
dan 160°C selama 3 jam serta ubi jalar cilembu mentah. Akrilamida pada
sampel dianalisis secara kualitatif dengan metode KLT dan kuantitatif
dengan metode spektrofotometri UV-Vis. Ubi jalar cilembu melalui
beberapa variasi suhu yang diekstraksi dengan pelarut dikloromethana
p.a dan dilarutkan dengan aquadest. Sampel di baca pada panjang
gelombang 206 nm. Hasil data dianalisis dengan menggunakan metode
one-way anova.
Hasil analisis kadar akrilamida pada ubi jalar cilembu mentah
sebesar 5,44 mg/Kg, sedangkan pada ubi jalar cilembu yang melalui
pemanasan suhu 100; 120; 140; dan 160°C sebesar 8,43; 20,09; 38,44;
dan 43,38 mg/Kg. Pada analisis spss menghasilkan perbedaan kadar
akrilamida yang signifikan pada semua variasi suhu. Berdasarkan hasil
penelitian variasi suhu pemanasan mempengaruhi kadar akrilamida yang
terbentuk sehingga semakin tinggi suhu pemanasan akan meningkatkan
kadar akrilamida.
Acrylamide is a carcinogenic compound, the mechanism is
formed by the reaction between protein (amino acids) and high
carbohydrates (reducing sugars) through a high temperature heating
process Cilembu sweet potato contains high protein and carbohydrates,
if it is processed by heating at high temperatures it will have the potential
to form acrylamide. The purpose of this study was to determine the effect
of temperature variations on acrylamide levels formed in cilembu sweet
potato.
Cilembu sweet potato samples were baked in a temperature
variation of 100; 120; 140; and 160°C for 3 hours and raw Cilembu sweet
potato. Acrylamide in the samples was analyzed qualitatively by TLC
method and quantitatively by UV-Vis spectrophotometry method.
Cilembu sweet potato through several temperature variations extracted
with dichloromethane p.a solvent and dissolved with distilled water. The
sample is read at a wavelength of 206 nm. The results of the data were
analyzed using the one-way ANOVA method.
The results of the analysis of acrylamide levels in raw Cilembu
sweet potato were 5.44 mg/Kg, while Cilembu sweet potatoes were
heated to a temperature of 100; 120; 140; and 160°C of 8.43; 20.09;
38.44; and 43.38 mg/Kg. The SPSS analysis yielded significant
differences in acrylamide levels at all temperature variations. Based on
the research results, variations in heating temperature affect the levels of
acrylamide formed, so that the higher the heating temperature, the higher
the acrylamide levels.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Akrilamida, ubi jalar cilembu, suhu, spektrofotometri UV-Vis. Acrylamide, cilembu sweet potato, temperature, UV-Vis spectrophotometry |
| Subjects: | R Medicine > RA Public aspects of medicine R Medicine > RB Pathology R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Universitas Setia Budi > Fakultas Farmasi > S1 Farmasi |
| Depositing User: | Unnamed user with email baa.si@setiabudi.ac.id |
| Date Deposited: | 04 Mar 2026 02:55 |
| Last Modified: | 04 Mar 2026 02:55 |
| URI: | https://eprints.setiabudi.ac.id/id/eprint/535 |
